6CIH
Crystal structure of a group II intron lariat in the post-catalytic state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1052 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 163.639, 255.361, 136.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.820 - 3.676 |
| R-factor | 0.2112 |
| Rwork | 0.208 |
| R-free | 0.25520 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.830 | 3.740 |
| High resolution limit [Å] | 3.676 | 3.676 |
| Number of reflections | 31107 | |
| <I/σ(I)> | 6.4 | 0.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 300 | 0.4 mM spermine, 21% 2-Methyl-2,4-pentanediol, 175 mM magnesium acetate tetrahydrate, and 90 mM MES monohydrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 303K, soaked in 3mM iridium hexammine |
