6CIG
CRYSTAL STRUCTURE ANALYSIS OF SELENOMETHIONINE SUBSTITUTED ISOFLAVONE O-METHYLTRANSFERASE
Replaces: 1FPXExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 1999-12-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97460 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.810, 50.240, 63.740 |
Unit cell angles | 90.00, 106.76, 90.00 |
Refinement procedure
Resolution | 39.280 - 1.650 |
R-factor | 0.1475 |
Rwork | 0.146 |
R-free | 0.17390 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.585 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.034 | 0.300 |
Number of reflections | 52130 | |
<I/σ(I)> | 15.6 | |
Completeness [%] | 97.6 | 95.2 |
Redundancy | 6 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 288 | Protein in 25 mM HEPES (pH 7.5), 100 mM NaCl, 1 mM DTT was mixed at 1:1 ratio with the crystallization buffer 17% (w/v) PEG 8000, 0.05 M TAPS (pH 8.25), 0.35 M lithium sulfate, 2 mM DTT |
1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 288 | Protein in 25 mM HEPES (pH 7.5), 100 mM NaCl, 1 mM DTT was mixed at 1:1 ratio with the crystallization buffer 17% (w/v) PEG 8000, 0.05 M TAPS (pH 8.25), 0.35 M lithium sulfate, 2 mM DTT |
1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 288 | Protein in 25 mM HEPES (pH 7.5), 100 mM NaCl, 1 mM DTT was mixed at 1:1 ratio with the crystallization buffer 17% (w/v) PEG 8000, 0.05 M TAPS (pH 8.25), 0.35 M lithium sulfate, 2 mM DTT |