6CI4
Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis soaked with UDP-4-amino-4,6-dideoxy-L-AltNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.425, 73.780, 41.805 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 46.885 - 1.824 |
| R-factor | 0.188269814514 |
| Rwork | 0.186 |
| R-free | 0.21113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ci2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.217 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.890 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rpim | 0.398 | |
| Number of reflections | 23891 | 1025 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 93.9 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 28.4% PEG5000 MME |
