6CI2

Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-13
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	95.630, 77.510, 41.730
Unit cell angles	90.00, 103.84, 90.00

Refinement procedure

Resolution	31.330 - 1.960
R-factor	0.1842
Rwork	0.180
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5es6
RMSD bond length	0.017
RMSD bond angle	1.329
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3042)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.500	2.000
High resolution limit [Å]	1.960	1.960
Rmerge	0.046	0.124
Number of reflections	21304
<I/σ(I)>	8
Completeness [%]	99.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	295	0.2 M ammonium sulfate, 0.1 M MES pH 6.6, 30.67% PEG5000 MME