6CI1
The Structure of Full-Length Kv Beta 2.1 Determined by Cryogenic Electron Microscopy
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | DIRECT ELECTRON DE-20 (5k x 3k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.9 |
| Software | MDFF,UCSF Chimera |
| Symmetry | D4 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
