6CHF

Crystal structure of a Fc fragment LALA mutant (L234A, L235A) of human IgG1 (crystal form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.905, 73.809, 136.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.055 - 2.400
R-factor	0.2185
Rwork	0.216
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.010
RMSD bond angle	1.269
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.319	0.600
Rpim	0.171	0.469
Number of reflections	18034	741
<I/σ(I)>	7.8
Completeness [%]	89.1	74.7
Redundancy	3.4	1.9
CC(1/2)		0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	20% PEG MME 5,000 0.1 M Bis-Tris pH 6.5