6CH3

Crystal structure of the cytoplasmic domain of FlhA and FliS-FliC complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	73.298, 88.623, 117.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.982 - 2.680
R-factor	0.2345
Rwork	0.233
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.458
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_3126: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.780
High resolution limit [Å]	2.680	2.680
Rmerge	0.096	1.130
Rmeas	0.106	1.300
Rpim	0.045	0.636
Number of reflections	21804	7894
<I/σ(I)>	15.7
Completeness [%]	98.7	91.74
Redundancy	5.3	3.9
CC(1/2)	0.998	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	tascimate