6CH2

Crystal structure of the cytoplasmic domain of FlhA and FliT-FliD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-07-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	49.110, 77.590, 119.270
Unit cell angles	86.88, 89.00, 84.27

Refinement procedure

Resolution	39.695 - 2.700
R-factor	0.2348
Rwork	0.233
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a5i
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2747: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.695	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.054	0.740
Number of reflections	44673
<I/σ(I)>	9.16	1.1
Completeness [%]	93.0	93.68
Redundancy	2.1	2.1
CC(1/2)	0.999	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	CaCl2, PEG8000, Sodium cacodylate