6CH1

Crystal structure of the cytoplasmic domain of FlhA in monomeric form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	40.380, 49.970, 50.950
Unit cell angles	78.04, 68.99, 80.66

Refinement procedure

Resolution	19.920 - 1.900
R-factor	0.2
Rwork	0.198
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a5i
RMSD bond length	0.004
RMSD bond angle	0.604
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((DEV_2706: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	193.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.046
Number of reflections	27461	1391
<I/σ(I)>	12.7	1.1
Completeness [%]	96.2	95.2
Redundancy	2.6	2.3
CC(1/2)	0.998	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	tri-ammonium citrate, PEG3350