6CGI
Structure of Salmonella Effector SseK3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-29 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.540, 96.170, 90.710 |
| Unit cell angles | 90.00, 93.28, 90.00 |
Refinement procedure
| Resolution | 48.360 - 2.300 |
| R-factor | 0.2188 |
| Rwork | 0.217 |
| R-free | 0.25020 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.813 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.360 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.110 | 0.595 |
| Number of reflections | 56770 | 16899 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5M NaCl, 0.1M Tris pH8.5, 18% PEG 3350 5%MPD |
