6CGB

chimera of mouse cadherin-11 EC1 and mouse cadherin-6 EC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792
Spacegroup name	I 21 21 21
Unit cell lengths	53.496, 81.030, 166.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.681 - 2.994
R-factor	0.2718
Rwork	0.270
R-free	0.30050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2A4C & 6CGU
RMSD bond length	0.004
RMSD bond angle	0.589
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.180
High resolution limit [Å]	2.994	2.994
Rmerge	0.124	1.766
Number of reflections	7540	720
<I/σ(I)>	14.8
Completeness [%]	99.9
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295.15	4 M sodium chloride, 0.1 M sodium acetate, pH 5.5, cryoprotectant: 30% v/v glycerol