6CG8
Structure of C. crescentus GapR-DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2017-12-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.913, 35.579, 115.559 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 57.779 - 2.299 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.27710 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.690 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.800 | 2.340 |
| High resolution limit [Å] | 2.299 | 2.299 |
| Rpim | 0.071 | 0.267 |
| Number of reflections | 32492 | 3035 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.977 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 400, CaCl2, HEPES |
