6CFR

Structure of Human alpha-Phosphomannomutase 1 containing mutation R183I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97917
Spacegroup name	P 43 21 2
Unit cell lengths	51.910, 51.910, 216.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.110 - 2.070
R-factor	0.1736
Rwork	0.169
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fuc
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.110	2.126
High resolution limit [Å]	2.070	2.070
Number of reflections	18258
<I/σ(I)>	9.08
Completeness [%]	96.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	20% polyethylene glycol 3350, 0.15 M DL-malate, pH 7.0, 50 mM MgCl2, and 8 mM beta-mercaptoethanol