6CFL

Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	210.150, 449.330, 623.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	224.665 - 2.600
R-factor	0.2111
Rwork	0.209
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.005
RMSD bond angle	0.887
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	224.665	224.665	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.187	0.037	1.734
Rmeas	0.202	0.041	1.882
Total number of observations	12571612
Number of reflections	1780463	20615	130600
<I/σ(I)>	9.49	35.86	1.11
Completeness [%]	99.9	98	99.4
Redundancy	7.061	6.774	6.621
CC(1/2)	0.994	0.997	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME