6CFI

Crystal structure of Rad4-Rad23 bound to a 6-4 photoproduct UV lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 18-ID
Synchrotron site	APS
Beamline	18-ID
Temperature [K]	103
Detector technology	CCD
Collection date	2010-10-28
Detector	MAR CCD 130 mm
Wavelength(s)	0.97938
Spacegroup name	P 41 21 2
Unit cell lengths	80.201, 80.201, 405.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.566 - 3.362
R-factor	0.221283344424
Rwork	0.219
R-free	0.26822
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12rc1_2801)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.950	3.483
High resolution limit [Å]	3.362	3.362
Rmerge	0.232
Rmeas	0.241
Rpim	0.064
Number of reflections	19112	1407
<I/σ(I)>	11.98
Completeness [%]	95.9	72.75
Redundancy	13.8	8.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	bis-tris propane 5 mM sodium chloride 100 mM 1-propanol 14% spermidine-HCl 5 mM dithiothreitol 5 mM