6CFF
Stimulator of Interferon Genes Human
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.930, 110.930, 36.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.090 - 2.396 |
| R-factor | 0.19833 |
| Rwork | 0.193 |
| R-free | 0.24918 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.457 |
| High resolution limit [Å] | 2.396 | 2.396 |
| Rmerge | 0.089 | 0.810 |
| Rmeas | 0.100 | |
| Rpim | 0.048 | 0.330 |
| Number of reflections | 8466 | |
| <I/σ(I)> | 9.5 | 2.4 |
| Completeness [%] | 97.9 | 99.3 |
| Redundancy | 4.3 | 4.5 |
| CC(1/2) | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 285 | Sodium citrate tribasic, PEG 3350, pH 8.2 |
