6CF9
Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni at 2.3A resolution in I23 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97940 |
Spacegroup name | I 2 3 |
Unit cell lengths | 178.674, 178.674, 178.674 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 126.340 - 2.290 |
R-factor | 0.2014 |
Rwork | 0.200 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cf8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.286 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.340 | 2.350 |
High resolution limit [Å] | 2.280 | 2.280 |
Number of reflections | 42902 | |
<I/σ(I)> | 21.5 | |
Completeness [%] | 99.6 | |
Redundancy | 40.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.2 M Ammonium acetate, 0.1 M sodium acetate pH 4.6, and 30% (w/v) PEG 4000 |