6CF8
Crystal structure of Cj0843 lytic transglycosylase of Campylobacter jejuni at 1.87A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.640, 74.640, 194.086 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.700 - 1.870 |
R-factor | 0.1934 |
Rwork | 0.191 |
R-free | 0.24170 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.292 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.700 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 51659 | |
<I/σ(I)> | 16.3 | |
Completeness [%] | 98.3 | |
Redundancy | 19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2 M lithium sulfate, 0.1 M Tris pH 8.0, and 39% PEG 400 |