6CF6
RNF146 TBM-Tankyrase ARC2-3 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99995 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.019, 103.284, 75.163 |
Unit cell angles | 90.00, 106.93, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.930 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3utm |
RMSD bond length | 0.010 |
RMSD bond angle | 1.340 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.990 |
High resolution limit [Å] | 1.930 | 4.420 | 1.930 |
Rmerge | 0.054 | 0.025 | 0.465 |
Rmeas | 0.063 | 0.030 | 0.582 |
Rpim | 0.033 | 0.015 | 0.344 |
Number of reflections | 71212 | 6188 | 4526 |
<I/σ(I)> | 11.8 | ||
Completeness [%] | 97.2 | 99.8 | 74.3 |
Redundancy | 3.6 | 3.7 | 2.3 |
CC(1/2) | 0.999 | 0.775 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30 mM sodium citrate pH 5.6, 60 mM ammonium acetate, 27% 2-methyl-2,4-pentanediol |