6CF2

Crystal structure of HIV-1 Rev (residues 1-93)-RNA aptamer complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	I 1 2 1
Unit cell lengths	64.190, 97.680, 87.836
Unit cell angles	90.00, 109.70, 90.00

Refinement procedure

Resolution	38.000 - 3.000
Rwork	0.208
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5DHV & 1ULL
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.088	0.634
Rmeas	0.124	0.897
Rpim	0.088	0.634
Number of reflections	10201
<I/σ(I)>	7.1	1.7
Completeness [%]	99.2	99.2
Redundancy	2	2
CC(1/2)	0.975	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	2% v/v 1,4-dioxane, 0.1 M Tris, pH 8.0, 15% w/v PEG3350