6CF2
Crystal structure of HIV-1 Rev (residues 1-93)-RNA aptamer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 64.190, 97.680, 87.836 |
| Unit cell angles | 90.00, 109.70, 90.00 |
Refinement procedure
| Resolution | 38.000 - 3.000 |
| Rwork | 0.208 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 5DHV & 1ULL |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.088 | 0.634 |
| Rmeas | 0.124 | 0.897 |
| Rpim | 0.088 | 0.634 |
| Number of reflections | 10201 | |
| <I/σ(I)> | 7.1 | 1.7 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.975 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.15 | 2% v/v 1,4-dioxane, 0.1 M Tris, pH 8.0, 15% w/v PEG3350 |
