6CEL
CBH1 (E212Q) CELLOPENTAOSE COMPLEX
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1995-08-31 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 83.050, 82.950, 110.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 1.700 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23500 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.900 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.083 | 0.408 |
Number of reflections | 42894 | |
<I/σ(I)> | 7.8 | 1.8 |
Completeness [%] | 93.5 | 90.9 |
Redundancy | 4.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | MES | 100 (mM) | |
3 | 1 | reservoir | mPEG5000 | 18 (%(w/v)) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | azide | 0.02 (%(w/v)) |