6CDW
Structure of the HAD domain of effector protein Lem4 (lpg1101) from Legionella pneumophila (inactive mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 105.730, 105.730, 37.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.284 - 1.840 |
R-factor | 0.1803 |
Rwork | 0.178 |
R-free | 0.21420 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.284 | 47.284 | 1.890 |
High resolution limit [Å] | 1.840 | 8.230 | 1.840 |
Rmerge | 0.124 | 0.050 | 1.838 |
Rmeas | 0.128 | 0.052 | 1.902 |
Total number of observations | 284030 | ||
Number of reflections | 18961 | 268 | 1374 |
<I/σ(I)> | 13.73 | 38.34 | 1.89 |
Completeness [%] | 100.0 | 99.3 | 100 |
Redundancy | 14.98 | 12.026 | 15.248 |
CC(1/2) | 0.999 | 0.999 | 0.825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1 M MES pH 6.5, 0.2 M magnesium acetate tetrahydrate, 0.75 M NaCl and 16% (w/v) PEG 8000 |