6CDD

Npl4 zinc finger and MPN domains (Chaetomium thermophilum)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.283
Spacegroup name	P 1 21 1
Unit cell lengths	58.858, 72.221, 193.543
Unit cell angles	90.00, 96.71, 90.00

Refinement procedure

Resolution	96.108 - 2.582
R-factor	0.193296283219
Rwork	0.192
R-free	0.22861
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	cryo-EM density
RMSD bond length	0.002
RMSD bond angle	0.529
Data scaling software	XDS
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.110	2.675
High resolution limit [Å]	2.582	2.582
Rmerge	0.064	2.031
Rpim	0.044	1.443
Number of reflections	96089	7754
<I/σ(I)>	9.82
Completeness [%]	95.2	77.79
Redundancy	2.9	2.7
CC(1/2)	0.998	0.322

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.2 M Na/K phosphate pH 7, 9.2% wt/vol poly-gamma-glutamic acid, 0.2 M potassium thiocyanate, 5% propylene glycol