6CC3

Crystal structure of ykoY-mntP riboswitch chimera bound to cadmium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.99
Spacegroup name	C 1 2 1
Unit cell lengths	125.531, 68.288, 83.384
Unit cell angles	90.00, 97.96, 90.00

Refinement procedure

Resolution	46.658 - 2.698
R-factor	0.2011
Rwork	0.196
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y1i
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.698	2.698
Rmerge	0.110	0.802
Number of reflections	19403	877
<I/σ(I)>	15.63
Completeness [%]	99.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	100 mM Magnesium Formate, 15% PEG 3350, 5mM Cadmium Chloride