6CBT
DnaG Primase C-terminal domain complex with SSB C-terminal peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.953715 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.284, 115.841, 45.742 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.742 - 2.100 |
R-factor | 0.2017 |
Rwork | 0.200 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t3w |
RMSD bond length | 0.002 |
RMSD bond angle | 0.546 |
Data reduction software | MOSFLM (7.0.4) |
Data scaling software | SCALA (3.3.15) |
Phasing software | MOLREP (9.4.09) |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.133 | 0.560 |
Rmeas | 0.146 | 0.610 |
Rpim | 0.057 | 0.230 |
Number of reflections | 21786 | 3075 |
<I/σ(I)> | 9 | 3.1 |
Completeness [%] | 96.8 | 95.7 |
Redundancy | 6.3 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 9.5% w/v PEG3000, 10 mM zinc acetate, 100 mM sodium acetate, pH 4.6 |