6CBT

DnaG Primase C-terminal domain complex with SSB C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953715
Spacegroup name	P 21 21 2
Unit cell lengths	70.284, 115.841, 45.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.742 - 2.100
R-factor	0.2017
Rwork	0.200
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3w
RMSD bond length	0.002
RMSD bond angle	0.546
Data reduction software	MOSFLM (7.0.4)
Data scaling software	SCALA (3.3.15)
Phasing software	MOLREP (9.4.09)
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.133	0.560
Rmeas	0.146	0.610
Rpim	0.057	0.230
Number of reflections	21786	3075
<I/σ(I)>	9	3.1
Completeness [%]	96.8	95.7
Redundancy	6.3	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	9.5% w/v PEG3000, 10 mM zinc acetate, 100 mM sodium acetate, pH 4.6