6CBR

DnaG Primase C-terminal domain complex with SSB C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95368
Spacegroup name	P 1
Unit cell lengths	31.723, 47.363, 51.881
Unit cell angles	63.51, 76.97, 73.81

Refinement procedure

Resolution	41.496 - 1.500
R-factor	0.1625
Rwork	0.162
R-free	0.17870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3w
RMSD bond length	0.007
RMSD bond angle	0.892
Data reduction software	iMOSFLM (6.2.6)
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP (9.4.09)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.039	0.275
Rmeas		0.389
Rpim	0.039	0.275
Number of reflections	41299	5369
<I/σ(I)>	13.5	2.4
Completeness [%]	90.0	88.6
Redundancy	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	10% w/v PEG3000, 5 mM zinc acetate, 5 mM 1,10-phenanthroline, 100 mM sodium acetate, pH 4.6