6CB9

Segment AALQSS from the low complexity domain of TDP-43, residues 328-333

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	4.790, 15.970, 42.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.220 - 1.100
R-factor	0.1994
Rwork	0.195
R-free	0.24300
RMSD bond length	0.010
RMSD bond angle	1.422
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	21.220	21.220	1.140
High resolution limit [Å]	1.100	4.120	1.100
Rmerge	0.211	0.141	0.562
Rmeas	0.230	0.161	0.605
Number of reflections	1432	39	119
<I/σ(I)>	5.12	6.35	2.82
Completeness [%]	90.7	100	72.1
Redundancy	6.853	3.821	7.252
CC(1/2)	0.970	0.973	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.01 M Nickel Chloride hexahydrate, 0.1 M Tris pH 8.5, 20% (w/v) PEG 2000