6CB0
Crystal Structure of the FAK FERM domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.152, 123.902, 135.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.970 |
| R-factor | 0.17321 |
| Rwork | 0.171 |
| R-free | 0.21205 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2al6 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.788 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.900 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.052 | 0.832 |
| Number of reflections | 55766 | 6816 |
| <I/σ(I)> | 21.5 | 1.99 |
| Completeness [%] | 92.4 | 78.5 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 0.2 M K/Na tartrate, 20% PEG 3,350 |
