6CAO

Structure of the ribosomal decoding complex at ambient temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.300, 402.300, 176.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.223 - 3.450
R-factor	0.219
Rwork	0.219
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.851
Data reduction software	CrystFEL
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.223	3.632
High resolution limit [Å]	3.450	3.450
Number of reflections	188443
<I/σ(I)>	5.89
Completeness [%]	100.0
Redundancy	384.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD