6CAM
Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-29 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.020, 47.940, 40.089 |
| Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
| Resolution | 46.720 - 1.750 |
| R-factor | 0.20834 |
| Rwork | 0.207 |
| R-free | 0.23474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uqz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.642 |
| Data reduction software | XDS (20170720) |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.720 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.153 | |
| Rpim | 0.094 | 0.529 |
| Number of reflections | 39634 | 2097 |
| <I/σ(I)> | 8.6 | 2.7 |
| Completeness [%] | 98.5 | 96.5 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.990 | 0.625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 295 | 25% PEG 3350, 0.1M citrate |
