6CA8

Crystal structure of Plasmodium falciparum topoisomerase II DNA-binding, cleavage and re-ligation domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	94
Detector technology	CCD
Collection date	2013-10-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.99
Spacegroup name	P 21 21 2
Unit cell lengths	83.034, 104.482, 114.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.218 - 2.331
R-factor	0.1894
Rwork	0.188
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.186
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.410
High resolution limit [Å]	2.330	2.330
Number of reflections	42437
<I/σ(I)>	15.6
Completeness [%]	98.0
Redundancy	26.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M Sodium formate, 19% PEG3350