6CA7
Crystal structure of PCT64_13C, a strain specific anti-HIV antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.091, 68.848, 78.502 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 42.971 - 1.643 |
| R-factor | 0.1703 |
| Rwork | 0.169 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5feh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.891 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rpim | 0.040 | 0.240 |
| Number of reflections | 55491 | |
| <I/σ(I)> | 19.6 | |
| Completeness [%] | 99.4 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 10% 2-propanol, 10% glycerol, 0.1 HEPES pH 7.5, and 20% PEG 2000 |
