6C9W
Crystal Structure of a ligand bound LacY/Nanobody Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2014-09-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1150 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 153.550, 153.550, 193.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.757 - 3.000 |
R-factor | 0.277 |
Rwork | 0.276 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zyr |
RMSD bond length | 0.005 |
RMSD bond angle | 0.725 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.757 | 40.000 | 3.500 |
High resolution limit [Å] | 3.000 | 30.000 | 3.000 |
Rmerge | 0.195 | 0.014 | 3.889 |
Rmeas | 0.204 | 0.015 | 4.060 |
Number of reflections | 27589 | 17 | 10024 |
<I/σ(I)> | 13.95 | 105.37 | 0.83 |
Completeness [%] | 99.8 | 68 | 99.9 |
Redundancy | 11.856 | 6.765 | 12.111 |
CC(1/2) | 0.999 | 1.000 | 0.514 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 295 | 0.1 M MgCl2, 28% PEG1000, 0.1 M Tris-HCl, pH 8.5 |