6C9T
Transcriptional repressor, CouR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 41 |
| Unit cell lengths | 59.008, 59.008, 108.402 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.010 - 2.070 |
| R-factor | 0.27508 |
| Rwork | 0.272 |
| R-free | 0.33441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c2s |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.707 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.010 | 2.108 |
| High resolution limit [Å] | 2.073 | 2.073 |
| Rmerge | 0.066 | 0.946 |
| Rmeas | 0.070 | 1.007 |
| Rpim | 0.024 | 0.343 |
| Number of reflections | 22136 | |
| <I/σ(I)> | 21 | 2.4 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 8.5 | 8.6 |
| CC(1/2) | 0.999 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 282 | 60% Tacsimate (v/v) pH 7.0 |
