6C9L
MEF2B Apo Protein Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.780, 178.490, 43.410 |
| Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
| Resolution | 89.240 - 2.300 |
| R-factor | 0.21577 |
| Rwork | 0.215 |
| R-free | 0.23482 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.521 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.250 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.331 |
| Number of reflections | 23268 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 98.0 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.4 M NaCl, 0.1 M Bis-Tris pH 5.5, 20% PEG3350 |
