6C8T
The structure of MppP soaked with the substrate L-Arg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.792, 108.425, 196.219 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.055 - 2.200 |
R-factor | 0.1475 |
Rwork | 0.146 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dj1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.119 |
Data reduction software | HKL-2000 ((1.11.1_2575: ???)) |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.057 | 0.193 |
Number of reflections | 85820 | 3839 |
<I/σ(I)> | 24.7 | 6.1 |
Completeness [%] | 91.6 | 84.1 |
Redundancy | 4.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 30% polyethylene glycol monomethyl ether 550, 50 mM MgCl2, and 0.1 M HEPES pH 8.0 |