6C8I

RNA-activated 2-AIpG monomer complex, 5 min soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2017-10-25
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	43.843, 43.843, 85.219
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.770
R-factor	0.23272
Rwork	0.230
R-free	0.28295
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dhc
RMSD bond length	0.025
RMSD bond angle	3.585
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.770	1.770
Rmerge	0.127	0.680
Rmeas	0.137	0.732
Rpim	0.051	0.265
Number of reflections	9699	965
<I/σ(I)>	14.19	1.95
Completeness [%]	99.1	99.8
Redundancy	7.6	7.2
CC(1/2)	0.947	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride hexahydrate