6C8H
Crystal structure of Transient Receptor Potential (TRP) channel TRPV4 in the presence of gadolinium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.7106 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 164.824, 164.824, 101.476 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 6.500 |
R-factor | 0.37335 |
Rwork | 0.373 |
R-free | 0.37301 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bbj |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 6.730 |
High resolution limit [Å] | 6.500 | 6.500 |
Rpim | 0.029 | 0.530 |
Number of reflections | 3026 | 296 |
<I/σ(I)> | 23.3 | |
Completeness [%] | 99.8 | |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM MES pH6.0, 100 mM NaCl, 5.5% PEG4000, and 10% glycerol |