6C8E
RNA-imidazolium-bridged intermediate complex, 4h soaking
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2017-04-04 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 49.023, 49.023, 81.317 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.460 - 1.800 |
R-factor | 0.26822 |
Rwork | 0.267 |
R-free | 0.28970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dhc |
RMSD bond length | 0.028 |
RMSD bond angle | 3.250 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.098 | 0.713 |
Rmeas | 0.103 | 0.752 |
Rpim | 0.033 | 0.236 |
Number of reflections | 10695 | 1051 |
<I/σ(I)> | 20.6 | 2.95 |
Completeness [%] | 97.7 | 100 |
Redundancy | 10.1 | 9.9 |
CC(1/2) | 0.979 | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride |