6C8D
RNA-dGMP complex with Mg ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2017-07-09 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 49.547, 49.547, 81.337 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.920 |
R-factor | 0.22252 |
Rwork | 0.219 |
R-free | 0.28184 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dhc |
RMSD bond length | 0.024 |
RMSD bond angle | 2.987 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.140 | 0.722 |
Rmeas | 0.153 | 0.772 |
Rpim | 0.056 | 0.271 |
Number of reflections | 9264 | 925 |
<I/σ(I)> | 12.6 | 2.64 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.9 | 8 |
CC(1/2) | 0.964 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride |