6C8C
Chimeric Pol kappa RIR Rev1 C-terminal domain in complex with JHRE06
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.977790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.550, 51.020, 98.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.171 - 1.500 |
| R-factor | 0.1651 |
| Rwork | 0.164 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c59 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.344 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.077 | 0.457 |
| Number of reflections | 40049 | 2888 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.73 | 4.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES pH 7.0, potassium chloride, 2-Methyl-2,4-pentanediol (MPD) beta-mercaptoethanol, PEG 3350, magnesium formate |
