6C87
Crystal structure of rab gdp dissociation inhibitor alpha from Naegleria fowleri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-02 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.340, 103.120, 195.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.950 - 2.600 |
R-factor | 0.1734 |
Rwork | 0.172 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cpi chain G as per Morda target identified with SIMBAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.950 | 47.950 | 2.670 |
High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
Rmerge | 0.075 | 0.025 | 0.545 |
Rmeas | 0.081 | 0.028 | 0.594 |
Number of reflections | 61169 | 752 | 4471 |
<I/σ(I)> | 19.54 | 46.94 | 3.42 |
Completeness [%] | 99.8 | 95.3 | 100 |
Redundancy | 6.228 | 5.158 | 6.307 |
CC(1/2) | 0.999 | 0.999 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Rigaku Reagents JCSG+ screen E1: 1000 mM Sodium citrate tribasic, 100mM Na cacodylate / HCl pH 6.5 : NafoA.01385.a.B1 at 21.13mg/ml: cryo: 20% EG: tray 295598 E1: puck meu4-5 |