6C80

Crystal structure of a flax cytokinin oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	CCD
Collection date	2012-05-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953691
Spacegroup name	I 1 2 1
Unit cell lengths	91.226, 58.929, 188.867
Unit cell angles	90.00, 95.62, 90.00

Refinement procedure

Resolution	49.355 - 1.780
R-factor	0.1523
Rwork	0.151
R-free	0.18370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2exr
RMSD bond length	0.007
RMSD bond angle	1.154
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.080	1.880
High resolution limit [Å]	1.780	1.780
Number of reflections	95714
<I/σ(I)>	2.8
Completeness [%]	99.8
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.24 M tribasic ammonium citrate (pH 8.0) and 18% (w/v) PEG-3350