6C7K
Crystal structure of an ACO/RPE65 chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979357 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.346, 125.663, 204.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.840 - 2.500 |
| R-factor | 0.18299 |
| Rwork | 0.181 |
| R-free | 0.21651 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4ou9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.196 | 1.752 |
| Number of reflections | 106564 | 16940 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 0.1 M Bis-Tris propane, pH 6 and 21% w/v sodium polyacrylate 2100 |
