6C74

Crystal Structure of Murine CD300lf in complex with phosphocholine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-10-29
Detector	NOIR-1
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	32.640, 40.058, 68.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.552 - 1.358
R-factor	0.1675
Rwork	0.165
R-free	0.18940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ffl
RMSD bond length	0.010
RMSD bond angle	1.274
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.020	40.020	1.370
High resolution limit [Å]	1.350	7.270	1.350
Rmerge	0.132	0.066	2.086
Rmeas	0.138	0.070	2.302
Rpim	0.039	0.021	0.944
Number of reflections	35969	171	870
<I/σ(I)>	10.6
Completeness [%]	98.4	99.6	86.6
Redundancy	11.8	10.9	5.6
CC(1/2)	0.998	0.984	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	293	0.1 M Phosphocholine, pH 7.4, 33% PEG3350