6C72

Crystal structure of a tailspike protein gp49 from Acinetobacter baumannii bacteriophage Fri1, a capsular polysaccharide depolymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	73.462, 98.664, 148.704
Unit cell angles	90.00, 91.81, 90.00

Refinement procedure

Resolution	49.332 - 2.396
R-factor	0.1569
Rwork	0.154
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in house model
RMSD bond length	0.006
RMSD bond angle	0.788
Data reduction software	XDS (March 30, 2013)
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.332	49.332	2.530
High resolution limit [Å]	2.396	7.580	2.400
Rmerge		0.024	0.405
Rmeas	0.095	0.037	0.510
Rpim	0.043	0.017	0.230
Total number of observations	388727
Number of reflections	81953	2711	11224
<I/σ(I)>	16	25.9	1.9
Completeness [%]	98.2	99.1	92.5
Redundancy	4.7	4.5	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	15% PEG 8000, 50 mM NaCl, 100 mM Tris HCl pH8.0