6C66
CRISPR RNA-guided surveillance complex, pre-nicking
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.66 |
| Number of Particle | 322268 |
| Software | SPIDER,PHENIX,RELION,UCSFImage4,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
