6C65
Crystal Structure of the Mango-II-A22U Fluorescent Aptamer Bound to TO1-Biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.722 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.639, 179.680, 108.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.381 - 2.800 |
| R-factor | 0.191169345205 |
| Rwork | 0.186 |
| R-free | 0.23689 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.225 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.381 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.102 | 0.956 |
| Number of reflections | 9216 | 1254 |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 11.3 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES pH 7.5, 10mM NiCl2, 2.3M Ammonium Formate, 12% glycerol |
