6C61
MHC-independent T-cell receptor B12A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.023, 43.630, 98.373 |
| Unit cell angles | 90.00, 125.85, 90.00 |
Refinement procedure
| Resolution | 39.720 - 2.430 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.24930 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | SCALEIT |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.470 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.125 | 0.385 |
| Number of reflections | 16078 | 603 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 95.0 | 74.8 |
| Redundancy | 6.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 4000, 0.1M sodium acetate, pH4.6, and 0.2M (NH4)2SO4 |
