6C5W

Crystal structure of the mitochondrial calcium uniporter

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	88.020, 290.450, 126.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.304 - 3.100
R-factor	0.2582532316
Rwork	0.256
R-free	0.29945
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	phenix.refine (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.304	3.160
High resolution limit [Å]	3.050	3.050
Rmeas	0.265
Number of reflections	30575	3009
<I/σ(I)>	11.1
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)		0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	15% PEG 2050, 200 mM (NH4)2SO4, and 100 mM Na-cacodylate, pH 6.0